human shh r d systems  (R&D Systems)

Journal: Developmental cell

Article Title: Phosphorylation of Ci/Gli by Fused family kinases promotes Hedgehog signaling

doi: 10.1016/j.devcel.2019.06.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Anti-Flag antibody Millipore Sigma Clone ID: M2; Cat#1804; RRID: AB_439685 Mouse Anti-HA (F-7) antibody Santa Cruz Biotechnology Cat#sc-7392; RRID: AB_627809 Mouse Anti-Myc antibody Santa Cruz Biotechnology Clone ID: 9E10; Cat#sc-40; RRID: AB_627268 Rabbit Anti-Myc antibody Abcam Cat#ab9106; RRID: AB_307014 Rat Anti-Ci antibody DSHB Cat#2A1; RRID: AB_2109711 Mouse Anti-Fu antibody DSHB Cat#22F10; RRID: AB_528258 Goat Anti-PKC ζ antibody Santa Cruz Biotechnology Clone ID: c-20; Cat#sc-216; RRID: AB_2300359 Mouse Anti-α-tubulin antibody Millipore Sigma Clone ID: DM1A; Cat#T9026; RRID: AB_477593 Mouse Anti-GFP antibody Takara Biol Cat#632380; RRID: AB_10013427 Mouse Anti-acetylated Tubulin antibody Millipore Sigma Clone ID:6-11b1; Cat#T7451; RRID: AB_609894 Goat Anti-Gli2 antibody R&D Systems Cat#AF3635; RRID: AB_2111902 Goat Anti-Gli3 antibody R&D Systems Cat#AF3690; RRID: AB_2232499 Bacterial and Virus Strains NEB5-Alpha Competent E. Coli New England Biolabs Cat# {"type":"entrez-nucleotide","attrs":{"text":"C29871","term_id":"2361667","term_text":"C29871"}} C29871 BL21 Competent E.Coli New England Biolabs Cat#C2530H Chemicals, Peptides, and Recombinant Proteins Leptomycin B Millipore Sigma Cat#L2913 Acti-Stain 670 phalloidin Cytoskeleton Cat#PHDN1-A Bluo-Gal Thermo Fisher Scientific Cat#15519028 L-Glutathione Reduced Millipore Sigma Cat#G4251 SAG Millipore Sigma Cat#566660 DAPI Millipore Sigma Cat#D9542 ATP [γ − 32 P] Perkin Elmer Cat#BLU002A Insulin Millipore Sigma Cat#I6634 Fetal Bovine Serum Millipore Sigma Cat#F4135 Fetal Bovine Serum Millipore Sigma Cat#F0926 Calf Bovine Serum Iron Fortified ATCC Cat#ATCC30-2030 3× Flag Peptide Millipore Sigma Cat#F4799 HA Synthetic Peptide Thermo Fisher Scientific Cat#26184 Casein Kinase I New England Biolabs Cat#P6030 Recombinant Human Sonic Hedgehog Protein (Shh-N) R&D Systems Cat#8908-HSH/CF Lambda Protein Phosphatase New England Biolabs Cat#P0753 Critical Commercial Assays Dual Luciferase Reporter Assay System Promega Cat#E1910 MEGAscript T7 High Yield Transcription Kit Thermo Fisher Scientific Cat#AM1334 pIMAGO-biotin Phosphoprotein Detection Kit Millipore Sigma Cat#67221 Anti-Flag M2 Affinity Gel Millipore Sigma Cat#A2220 Pierce Anti-HA Argarose Thermo Fisher Scientific Cat#26181 Glutathione Sepharose 4B GE Healthcare Cat#17075601 SimplyBlue SafeStain Thermo Fisher Scientific Cat#LC6060 RNeasy Plus Mini Kit Qiagen Cat#74134 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 Fast SYBR Green Master Mix Applied Biosystems Cat#4385612 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7572 Experimental Models: Cell Lines Mouse: NIH/3T3 ATCC Cat#ATCC CRL-1658 Human: HEK293T/17 ATCC Cat#ATCC CRL-11268 D. melanogaster .

Techniques: Virus, Recombinant, Luciferase, Reporter Assay, Reverse Transcription, SYBR Green Assay, Cell Viability Assay, Knock-In, Software