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human shh r d systems  (R&D Systems)


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    R&D Systems human shh r d systems
    Human Shh R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 116 article reviews
    human shh r d systems - by Bioz Stars, 2026-09
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    Recombinant:

    Article Title: Meningeal macrophages inhibit chemokine signaling in pre-tumor cells to suppress mouse medulloblastoma initiation.
    Article Snippet: N/A Mouse mIgG2A Roche. .. N/A Mouse anti-CD20 Genentech Inc. Cat# BE0356; RRID:AB_2894775 Mouse mIgG2A Genentech Inc. Cat# BE0366; RRID:AB_2894738 Biological samples Mouse cerebellar tissue This manuscript N/A Mouse cerebellar meningeal tissue This manuscript N/A Chemicals, peptides, and recombinant proteins Shh R&D Systems Cat# 464-SH-025/CF CXCL12/SDF1 R&D Systems Cat# 460-SD-010/CF CXCL4/PF4 R&D Systems Cat# 595-P4-025 AMD3100/Plerixafor SelleckChem Cat# S8030 PLX3397/Pexidartinib SelleckChem Cat# S7818 (Continued on next page) e1 Developmental Cell 58, 2015–2031.e1–e8, October 23, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TO-PRO-3 Iodide Thermo Fisher Scientific Cat# T3605 Dispase II Sigma Aldrich Cat# D4693-1G B-27 Supplement Thermo Fisher Scientific Cat# 12587010 Neurobasal A Medium Sigma Aldrich Cat# 10888022 L-Glutamine 200mM Gibco Cat# 25030-061 Penicillin/Streptomycin Thermo Fisher Scientific Cat# 15140122 KCl Sigma Aldrich Cat# 4504-500G Donkey Serum Sigma Aldrich Cat# D9663-10ML Fetal Bovine Serum Thermo Fisher Scientific Cat# 12483020 Poly-D-lysine hydrobromide Sigma Aldrich Cat# 7405-5MG Methanol Caledon Cat# 6701-7-40 Dichloromethane Caledon Cat# 3600-1-40 Dibenzyl Ether Sigma-Aldrich Cat# 33630-1L Acetone Sigma Aldrich Cat# 179124-4L Crystal Violet BioShop Cat# CRY422.25 DAKO fluorescent mounting medium Agilent Cat# S3023 Percoll Sigma Aldrich Cat# P1644-500ML Critical commercial assays Papain Dissociation System Worthington Cat# LK003150 RNAscope Intro Pack for Multiplex Fluorescent Reagent Kit ACDBio Cat# 323133 RNAscope 3-plex Negative Control Probe ACDBio Cat# 320871 RNAscope Probe - Mm-Pf4-C3 ACDBio Cat# 502391-C3 RNAscope Probe - Mm-Lyve1 ACDBio Cat# 428451 RNAscope Probe - Mm-Cxcr4-C3 ACDBio Cat# 425901-C3 RNAscope Probe - Mm-Cxcl12-C2 ACDBio Cat# 422711-C2 EdU (5-ethynyl-2’-deoxyuridine) ThermoFisher Scientific Cat# E10187 Alexa Fluor 647 Azide, Triethylammonium Salt ThermoFisher Scientific Cat# A10277 Alexa Fluor 555 Azide, Triethylammonium Salt ThermoFisher Scientific Cat# A20012 Alexa Fluor 488 Azide, Triethylammonium Salt ThermoFisher Scientific Cat# A10266 Deposited data Single cell RNA sequencing of post natal day 7 mouse cerebellar subdural meninges NCBI Accession number: GSE226805 Experimental models: Organisms/strains C57/BL6 The Jackson Laboratory RRID:IMSR_JAX:000664 Ptch+/- The Jackson Laboratory RRID:MGI:2177702 Pf4tm1Mako(CXCL4KO) The Jackson Laboratory RRID:MGI:3525230 Fzd4fl/fl The Jackson Laboratory RRID:MGI:4412187 NeuroD2-SmoA1+/- The Jackson Laboratory RRID:MGI:3831004 Ndp-/Y MGI RRID:MGI:4414648 Tie2-Cre+/- The Jackson Laboratory RRID:IMSR_JAX:008863 Oligonucleotides Ndp WT mouse Reverse 5’ CAG GGA GAG CAT AGA AAT GG 3’ This paper N/A Ndp KO mouse Reverse 5’ CCC TAG GAA TGC TCG TCA AGA 3’ This paper N/A Ndp mouse Forward 5’ TCT GAT TTC ATT CCA GCT GTG 3’ This paper N/A Ptch mouse Reverse 5’ TGG GGT GGG ATT AGA TAA ATG CC 3’ This paper N/A (Continued on next page) Developmental Cell 58, 2015–2031.e1–e8, October 23, 2023 e2



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    Journal: Developmental cell

    Article Title: Phosphorylation of Ci/Gli by Fused family kinases promotes Hedgehog signaling

    doi: 10.1016/j.devcel.2019.06.008

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Anti-Flag antibody Millipore Sigma Clone ID: M2; Cat#1804; RRID: AB_439685 Mouse Anti-HA (F-7) antibody Santa Cruz Biotechnology Cat#sc-7392; RRID: AB_627809 Mouse Anti-Myc antibody Santa Cruz Biotechnology Clone ID: 9E10; Cat#sc-40; RRID: AB_627268 Rabbit Anti-Myc antibody Abcam Cat#ab9106; RRID: AB_307014 Rat Anti-Ci antibody DSHB Cat#2A1; RRID: AB_2109711 Mouse Anti-Fu antibody DSHB Cat#22F10; RRID: AB_528258 Goat Anti-PKC ζ antibody Santa Cruz Biotechnology Clone ID: c-20; Cat#sc-216; RRID: AB_2300359 Mouse Anti-α-tubulin antibody Millipore Sigma Clone ID: DM1A; Cat#T9026; RRID: AB_477593 Mouse Anti-GFP antibody Takara Biol Cat#632380; RRID: AB_10013427 Mouse Anti-acetylated Tubulin antibody Millipore Sigma Clone ID:6-11b1; Cat#T7451; RRID: AB_609894 Goat Anti-Gli2 antibody R&D Systems Cat#AF3635; RRID: AB_2111902 Goat Anti-Gli3 antibody R&D Systems Cat#AF3690; RRID: AB_2232499 Bacterial and Virus Strains NEB5-Alpha Competent E. Coli New England Biolabs Cat# {"type":"entrez-nucleotide","attrs":{"text":"C29871","term_id":"2361667","term_text":"C29871"}} C29871 BL21 Competent E.Coli New England Biolabs Cat#C2530H Chemicals, Peptides, and Recombinant Proteins Leptomycin B Millipore Sigma Cat#L2913 Acti-Stain 670 phalloidin Cytoskeleton Cat#PHDN1-A Bluo-Gal Thermo Fisher Scientific Cat#15519028 L-Glutathione Reduced Millipore Sigma Cat#G4251 SAG Millipore Sigma Cat#566660 DAPI Millipore Sigma Cat#D9542 ATP [γ − 32 P] Perkin Elmer Cat#BLU002A Insulin Millipore Sigma Cat#I6634 Fetal Bovine Serum Millipore Sigma Cat#F4135 Fetal Bovine Serum Millipore Sigma Cat#F0926 Calf Bovine Serum Iron Fortified ATCC Cat#ATCC30-2030 3× Flag Peptide Millipore Sigma Cat#F4799 HA Synthetic Peptide Thermo Fisher Scientific Cat#26184 Casein Kinase I New England Biolabs Cat#P6030 Recombinant Human Sonic Hedgehog Protein (Shh-N) R&D Systems Cat#8908-HSH/CF Lambda Protein Phosphatase New England Biolabs Cat#P0753 Critical Commercial Assays Dual Luciferase Reporter Assay System Promega Cat#E1910 MEGAscript T7 High Yield Transcription Kit Thermo Fisher Scientific Cat#AM1334 pIMAGO-biotin Phosphoprotein Detection Kit Millipore Sigma Cat#67221 Anti-Flag M2 Affinity Gel Millipore Sigma Cat#A2220 Pierce Anti-HA Argarose Thermo Fisher Scientific Cat#26181 Glutathione Sepharose 4B GE Healthcare Cat#17075601 SimplyBlue SafeStain Thermo Fisher Scientific Cat#LC6060 RNeasy Plus Mini Kit Qiagen Cat#74134 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 Fast SYBR Green Master Mix Applied Biosystems Cat#4385612 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7572 Experimental Models: Cell Lines Mouse: NIH/3T3 ATCC Cat#ATCC CRL-1658 Human: HEK293T/17 ATCC Cat#ATCC CRL-11268 D. melanogaster .

    Techniques: Virus, Recombinant, Luciferase, Reporter Assay, Reverse Transcription, SYBR Green Assay, Cell Viability Assay, Knock-In, Software